Visualizing fungal metabolites during mycoparasitic interaction by MALDI mass spectrometry imaging

详细信息    查看全文

• 作者：Matthias Holzlechner ; Sonja Reitschmidt ; Sabine Gruber ; Susanne Zeilinger and Martina Marchetti-Deschmann
• 刊名：PROTEOMICS
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：16
• 期：11-12
• 页码：1742-1746
• 全文大小：467K
• ISSN：1615-9861

文摘

Studying microbial interactions by MALDI mass spectrometry imaging (MSI) directly from growing media is a difficult task if high sensitivity is demanded. We present a quick and robust sample preparation strategy for growing fungi (Trichoderma atroviride, Rhizoctonia solani) on glass slides to establish a miniaturized confrontation assay. By this we were able to visualize metabolite distributions by MALDI MSI after matrix deposition with a home-built sublimation device and thorough recrystallization. We present for the first time MALDI MSI data for secondary metabolite release during active mycoparasitism.