Schistosomicidal Effects of the Essential Oils of <em>Citrus limoniaem> and <em>Citrus reticulataem> Against <em>Schistosoma mansoniem>

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• 作者：Moara H. G. Martins ; Let&iacute ; cia Fracarolli ; Tatiana M. Vieira ; Herbert J. Dias ; Michele G. Cruz ; C&aacute ; ssia C. H. Deus ; Heloiza D. Nicolella ; Ricardo Stefani ; Vanderlei Rodrigues ; Denise C. Tavares ; Lizandra G. Magalh&atilde ; es and Antô ; nio E. M. Crotti
• 刊名：Chemistry & Biodiversity
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：14
• 期：1
• 全文大小：410K
• ISSN：1612-1880

文摘

We report the <em>in vitroem> schistosomicidal effects of the essential oil obtained from <em>Citrus limoniaem> leaves (CL-EO) and <em>C. reticulataem> fruit peels (CR-EO), cultivated in Brazil, against <em>Schistosoma mansoniem> worms. Limonene (29.9%), <em>&beta;em>-pinene (12.0%), sabinene (9.0%), citronellal (9.0%), and citronellol (5.8%) are the major constituents of CL-EO; limonene (26.5%), <em>γem>-terpinene (17.2%), linalool (11.1%), octanal (8.0%), myrcene (6.2%), and capraldehyde (3.9%) predominate in CR-EO. CL-EO displayed moderate lethal concentration 50% (<em>LCem>₅₀) of 81.7 and 38.9 μg/ml against male and female worms at 24 and 72 h, respectively. At concentrations of 25 and 100 μg/ml, CL-EO separated between 50 and 75% of the coupled worm pairs during the evaluated period. CR-EO presented moderate <em>LCem>₅₀ of 81.7 μg/ml against male and female worms at 24 and 72 h. However, this oil separated coupled worm pairs more effectively than CL-EO and displayed lower cytotoxicity to GM07492-A cells (<em>ICem>₅₀ = 987.7 &plusmn; 88.9 μg/ml) as compared to CL-EO (<em>ICem>₅₀ = 187.8 &plusmn; 2.9 μg/ml). The enantiomers (+)-(<em>Rem>)-limonene and (−)-(<em>Sem>)-limonene did not affect <em>S. mansoniem> adult worm pairs significantly. Taken together, these data indicate that CL-EO and CR-EO exhibit moderate <em>in vitroem> schistosomicidal activity against adult <em>S. mansoniem> worms.