Combined Use of Oligopeptides, Fragment Libraries, and Natural Compounds: A Comprehensive Approach To Sample the Druggability of Vascular Endothelial Growth Factor

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• 作者：Dr. Nú ; ria Bayó ; -Puxan ; Dr. Ricard Rodrí ; guez-Mias ; Dr. Michael Goldflam ; Dr. Martin Kotev ; Sonia Ciudad ; Dr. Christopher J. Hipolito ; Dr. Monica Varese ; Prof. Hiroaki Suga ; Dr. Ramó ; n Campos-Olivas ; Dr. Xavier Barril ; Prof. Ví ; ctor Guallar ; Dr. Meritxell Teixidó ; Dr. Jesú ; s Garcí ; a and Prof. Ernest Giralt
• 关键词：drug discovery ; fragment screening ; growth factors ; peptides ; protein&ndash ; protein interactions
• 刊名：ChemMedChem
• 出版年：2016
• 出版时间：April 19, 2016
• 年：2016
• 卷：11
• 期：8
• 页码：928-939
• 全文大小：2521K
• ISSN：1860-7187

文摘

The modulation of protein–protein interactions (PPIs) is emerging as a highly promising tool to fight diseases. However, whereas an increasing number of compounds are able to disrupt peptide-mediated PPIs efficiently, the inhibition of domain–domain PPIs appears to be much more challenging. Herein, we report our results related to the interaction between vascular endothelial growth factor (VEGF) and its receptor (VEGFR). The VEGF–VEGFR interaction is a typical domain–domain PPI that is highly relevant for the treatment of cancer and some retinopathies. Our final goal was to identify ligands able to bind VEGF at the region used by the growth factor to interact with its receptor. We undertook an extensive study, combining a variety of experimental approaches, including NMR-spectroscopy-based screening of small organic fragments, peptide libraries, and medicinal plant extracts. The key feature of the successful ligands that emerged from this study was their capacity to expose hydrophobic functional groups able to interact with the hydrophobic hot spots at the interacting VEGF surface patch.