Identification of a distinct subpopulation of fibroblasts from murine dermis: CD73−CD105+ as potential marker of dermal fibroblasts subset with multipotency

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• 作者：Seung Bum Lee ; Sehwan Shim ; Min-Jung Kim ; Hye-Yun Shin ; Won-Suk Jang ; Sun-Joo Lee ; Young-Woo Jin ; Seung-Sook Lee and Sunhoo Park
• 刊名：Cell Biology International
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：40
• 期：9
• 页码：1008-1016
• 全文大小：4176K
• ISSN：1095-8355

文摘

Skin dermis includes various types of multipotent stromal cells (MSCs) and a subpopulation of dermal fibroblasts that exhibit the ability to differentiate. However, characterization of this dermal fibroblast subtype remains less understood. In this study, we isolated dermal cells from the skin of newborn C57/B6 mice and investigated their characteristics. Isolated murine dermal cells exhibited a fibroblast phenotype as judged by accepted criteria including a lack of MSC-related antigens and the differentiation potential of MSCs, and the positive expression of fibroblast markers. A comparative analysis demonstrated that CD73^−CD105⁺ but not CD73^− CD105^− dermal fibroblasts exhibited some of the functional properties of MSCs. Furthermore, the multipotent phenotype of CD73^−CD105⁺ cells was diminished by treatment of CD105 siRNA and shRNA, indicating that CD105 expression was critical for the retention of differentiation potential of those cells. Overall, these results suggest that CD73^−CD105⁺ cells are a distinct subset of dermal fibroblasts with multipotency and that their surface antigens could help to classify this subpopulation. These cells may contribute to the regeneration of damaged tissue.