Intracellular Delivery of a Planar DNA Origami Structure by the Transferrin-Receptor Internalization Pathway

详细信息    查看全文

• 作者：David H. Schaffert ; Anders H. Okholm ; Rasmus S. Sø ; rensen ; Jesper S. Nielsen ; Thomas Tø ; rring ; Christian B. Rosen ; Anne Louise B. Kodal ; Michael R. Mortensen ; Kurt V. Gothelf and Jø ; rgen Kjems
• 刊名：Small
• 出版年：2016
• 出版时间：May 18, 2016
• 年：2016
• 卷：12
• 期：19
• 页码：2634-2640
• 全文大小：1854K
• ISSN：1613-6829

文摘

DNA origami provides rapid access to easily functionalized, nanometer-sized structures making it an intriguing platform for the development of defined drug delivery and sensor systems. Low cellular uptake of DNA nanostructures is a major obstacle in the development of DNA-based delivery platforms. Herein, significant strong increase in cellular uptake in an established cancer cell line by modifying a planar DNA origami structure with the iron transport protein transferrin (Tf) is demonstrated. A variable number of Tf molecules are coupled to the origami structure using a DNA-directed, site-selective labeling technique to retain ligand functionality. A combination of confocal fluorescence microscopy and quantitative (qPCR) techniques shows up to 22-fold increased cytoplasmic uptake compared to unmodified structures and with an efficiency that correlates to the number of transferrin molecules on the origami surface.