MC3T3-E1 Cells Behavior on Surfaces Bombarded by Argon Ions in Planar Cathode Discharge

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• 作者：Carlos Eduardo Bezerra Moura ; Naisandra Bezerra Silva ; Juliana Carvalho Sa ; Geraldo Barroso Cavalcanti Junior ; Silvia Regina Batistuzzo de Medeiros ; Hugo Alexandre Oliveira Rocha ; Paula Carvalho Papa and Clodomiro Alves Jr
• 刊名：Artificial Organs
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：40
• 期：5
• 页码：497-504
• 全文大小：334K
• ISSN：1525-1594

文摘

To evaluate the effect of topography in nanoscale, titanium surfaces were bombarded by argon ions (a chemically inert gas), in an atmosphere of plasma. The effects of surface parameters on morphology, adhesion, proliferation, and MC3T3-E1 preosteoblasts differentiation were analyzed. Nontreated (smooth) surfaces were used as a control. The levels of average roughness (Ra) observed in bombarded and smooth titanium surfaces were of 95 and 14 nm, respectively. The wettability increased on treated surfaces. The number of attached cells (30 and 60 min) was significantly higher on the bombarded surface. The cell proliferation after 3 and 7 days was also significantly higher on the ion-bombarded surface. In addition, the ALP activity and expression of osteocalcin were higher in cells grown on the treated surface. The results showed that bombardment with argon ions increased the roughness and the wettability of the Ti surface, promoting a significant increase in the adhesion, proliferation, and differentiation of preosteoblasts.