Distinct and shared three-dimensional chromosome organization patterns in lymphocytes, monoclonal gammopathy of undetermined significance and multiple myeloma
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- 作者：Chirawadee Sathitruangsak ; Christiaan H. Righolt ; Ludger Klewes ; Doris Tung Chang ; Rami Kotb and Sabine Mai
- 刊名：International Journal of Cancer
- 出版年：2017
- 出版时间：15 January 2017
- 年：2017
- 卷：140
- 期：2
- 页码：400-410
- 全文大小：575K
- ISSN：1097-0215
文摘
The consistent appearance of specific chromosomal translocations in multiple myeloma has suggested that the positioning of chromosomes in the interphase nucleus might play a role in the occurrence of particular chromosomal rearrangements associated with malignant transformation. Using fluorescence in situ hybridization, we have determined the positions of selected chromosome pairs (18 and 19, 9 and 22, 4 and 14, 14 and 16, 11 and 14) in interphase nuclei of myeloma cells compared to normal lymphocytes of treatment-naïve patients. All chromosome pairs were arranged in a nonrandom pattern. Chromosomes commonly involved in myeloma-associated translocations (4 and 14, 14 and 16, 11 and 14) were found in close spatial proximity, and this is correlated with the occurrence of overlapping chromosome territories. The spatial distribution of chromosomes may increase the possibility of chromosomal translocations in multiple myeloma.