DNA Release from a Bioelectronic Interface Stimulated by a DNA Signal - Amplification of DNA Signals

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• 作者：Maria Gamella ; Nataliia Guz and Evgeny Katz
• 刊名：Electroanalysis
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：28
• 期：11
• 页码：2692-2696
• 全文大小：1326K
• ISSN：1521-4109

文摘

A new bioelectronic system activated by a DNA-signal and releasing another DNA has been designed. The system was composed of two modified electrodes: one electrode functionalized with enzymes was activated with a DNA-signal, while another electrode coated with an alginate film released pre-loaded DNA species, when the first electrode was activated. The sensing electrode was functionalized with pyrroloquinoline quinone-dependent glucose dehydrogenase (PQQ-GDH) producing negative potential in the presence of glucose. This bioelectrocatalytic process was inhibited by the presence of glucose oxidase (GOx) at the external surface of the biomolecular system organized on the electrode surface through a DNA-linker. GOx consumed incoming glucose and did not allow its penetration to the internal layer functionalized with PQQ-GDH, thus leaving the electrode mute. The DNA-signal resulted in the detachment of GOx and activated the process biocatalyzed by PQQ-GDH due to penetration of glucose to the internal layer at the electrode surface. Finally, formation of a negative potential on the sensing electrode resulted in the activation of the second electrode and DNA release from the alginate matrix. The Sense-and-Act system allowed amplification of the DNA-signal by releasing much higher amount of DNA comparing with the applied DNA-signal. The DNA-signal amplification will find applications in various biosensor and biomolecular computing systems.