Self-Regulated Glucose-Sensitive Neoglycoenzyme-Capped Mesoporous Silica Nanoparticles for Insulin Delivery
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- 作者：Mar Oroval ; Paula Dí ; ez ; Dr. Elena Aznar ; Dr. Carmen Coll ; Dr. Marí ; a Dolores Marcos ; Dr. Fé ; lix Sancenó ; n ; Dr. Reynaldo Villalonga and Prof. Ramó ; n Martí ; nez-Má ; ñ ; ez
- 刊名：Chemistry - A European Journal
- 出版年：2017
- 出版时间：January 26, 2017
- 年：2017
- 卷：23
- 期：6
- 页码：1353-1360
- 全文大小：1310K
- ISSN：1521-3765
文摘
We describe herein the preparation of glucose-sensitive capped mesoporous silica nanoparticles for insulin delivery. The new material consists of an expanded-pore nanometric silica support grafted with 1-propyl-1-H-benzimidazole groups, loaded with fluorescein isothiocyanate-labeled insulin (FITC-Ins) and capped by the formation of inclusion complexes between cyclodextrin-modified glucose oxidase (CD-GOx) and the benzimidazole groups grafted on the mesoporous support. Insulin delivery from the gated material in simulated blood plasma was assessed upon addition of glucose. Glucose is transformed by GOx into gluconic acid, which promoted the dethreading of the benzimidazole–CD-GOx inclusion complexes, allowing cargo release. Small quantities of this support would be needed to release the amount of insulin necessary to decrease diabetic blood glucose concentrations to regular levels.