Residue level and dissipation pattern of lepimectin in shallots using high-performance liquid chromatography coupled with photodiode array detection

详细信息    查看全文

• 作者：Sung-Woo Kim ; Md. Musfiqur Rahman ; A. M. Abd El-Aty ; Lieu T. B. Truong ; Jeong-Heui Choi ; Joon-Seong Park ; Mi-Ra Kim ; Ho-Chul Shin and Jae-Han Shim
• 刊名：Biomedical Chromatography
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：30
• 期：11
• 页码：1835-1842
• 全文大小：475K
• ISSN：1099-0801

文摘

Lepimectin, as an emulsifiable concentrate, was sprayed on shallots at the recommended dose rate (10 mL/20 L) to determine its residue levels, dissipation pattern, pre-harvest residue limits (PHRLs), and health risk. Samples were randomly collected over 10 days, extracted with acetonitrile, purified using an amino solid-phase extraction (NH₂-SPE) cartridge and analyzed using a high-performance liquid chromatography–photodiode array detection method. Field-incurred samples were confirmed using ultra-performance liquid chromatography–tandem mass spectrometry. The linearity was excellent, with a determination coefficient (R²) of ≥0.9991. The recoveries at two spiking levels (0.2 and 1.0 mg/kg) ranged from 84.49 to 87.64% with relative standard deviations of ≤7.04%. The developed method was applied to field samples grown in separate greenhouses, one located in Naju and one in Muan, in the Republic of Korea. The dissipation pattern was described by first-order kinetics with half-lives of 1.9 (Naju) and 1.7 days (Muan). The PHRL curves indicated that, if the lepimectin residues are <0.18 (Naju) and <0.13 mg/kg (Muan) 5 days before harvest, the residue levels will be lower than the maximum residue limit (0.05 mg/kg) upon harvesting. The risk assessment data indicated that lepimectin is safe for use in the cultivation of shallots, with no risk of detrimental effects to the consumer.