Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography
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- 作者：Saeed Oghbaey ; Antoine Sarracini ; Helen M. Ginn ; Olivier Pare-Labrosse ; Anling Kuo ; Alexander Marx ; Sascha W. Epp ; Darren A. Sherrell ; Bryan T. Eger ; Yinpeng Zhong ; Rolf Loch ; Valerio Mariani ; Roberto Alonso-Mori ; Silke Nelson ; Henrik T. Lemke ; Robin L. Owen ; Arwen R. Pearson ; David I. Stuart ; Oliver P. Ernst ; Henrike M. Mueller-Werkmeister and R. J. Dwayne Miller
- 关键词：femtosecond time-resolved crystallography ; high-throughput serial crystallography ; fixed-target sample delivery ; room-temperature crystallography
- 刊名：Acta Crystallographica Section D
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：72
- 期：8
- 页码：944-955
- 全文大小：1885K
- ISSN：1399-0047
文摘
The advent of ultrafast highly brilliant coherent X-ray free-electron laser sources has driven the development of novel structure-determination approaches for proteins, and promises visualization of protein dynamics on sub-picosecond timescales with full atomic resolution. Significant efforts are being applied to the development of sample-delivery systems that allow these unique sources to be most efficiently exploited for high-throughput serial femtosecond crystallography. Here, the next iteration of a fixed-target crystallography chip designed for rapid and reliable delivery of up to 11 259 protein crystals with high spatial precision is presented. An experimental scheme for predetermining the positions of crystals in the chip by means of in situ spectroscopy using a fiducial system for rapid, precise alignment and registration of the crystal positions is presented. This delivers unprecedented performance in serial crystallography experiments at room temperature under atmospheric pressure, giving a raw hit rate approaching 100% with an effective indexing rate of approximately 50%, increasing the efficiency of beam usage and allowing the method to be applied to systems where the number of crystals is limited.