Quality control of extracorporeal photochemotherapy: Proliferation assay using CFSE validated according to ISO 15189:2007 standards

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• 作者：Lionel Faivre ; Lucie Lecouflet ; Wang-Qing Liu ; Isabelle Khadher ; Camille Lahaie ; Michel Vidal ; Sabine Legouvello ; Jean-Louis Beaumont ; Philippe Bierling ; Hé ; lè ; ne Rouard and Brigitte Birebent
• 关键词：CFSE ; lymphocyte proliferation ; biological assay ; quality control ; photochemotherapy
• 刊名：Cytometry Part B: Clinical Cytometry
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：88
• 期：1
• 页码：30-39
• 全文大小：322K
• ISSN：1552-4957

文摘

For the last 40 years, the technique of extracorporeal photopheresis (ECP) has constantly developed. Among irradiation systems, those called “off-line” allow the validation of the quality of the cell therapy product. The inhibition of the proliferation of lymphocytes after ultraviolet irradiation (UVA) is usually verified by the tritiated thymidine assay as in vitro proliferation assay. The document presented here describes the results obtained while performing the setting up of an alternative proliferation assay using flow cytometry according to ISO 15189:2007 Standard.