Evaluation of the influence of salt processing on pharmacokinetics of psoralen and isopsoralen in Psoralea corylifolia L.

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• 作者：Qian qian Gao ; Cui ping Yan ; Zi sheng Xu ; Yu Wu ; Ze bin Weng ; Gen hua Zhao ; Liu jie Zhang ; Jia yu He ; Bao chang Cai ; Zhi peng Chen and Wei dong Li
• 关键词：salt processing ; psoralen ; isopsoralen ; pharmacokinetics ; ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS)
• 刊名：Biomedical Chromatography
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：30
• 期：4
• 页码：528-535
• 全文大小：731K
• ISSN：1099-0801

文摘

A sensitive, specific and rapid ultra-high-pressure liquid chromatography tandem mass spectrometry (UHPLC-MS/MS) method has been developed to investigate pharmacokinetic properties of psoralen and isopsoralen, two compounds isolated from raw/salt-processed fruit of Psoralea corylifolia L. UHPLC-MS/MS was used with positive ion electrospray. The mobile phase was composed of acetonitrile and 0.1% formic acid aqueous solution and a gradient elution program at flow rate of 0.3 mL/min was applied. Multiple reaction monitoring mode was used for the quantification of psoralen, isopsoralen ([M + H]⁺ m/z 187.0  m/z 131.0) and scoparone (m/z 207.0  m/z 151.1). Scoparone served as an internal standard. The method was fully validated for its sensitivity, selectivity, stability, matrix effect and extraction recovery. The obtained results showed that salt-processed Buguzhi significantly promoted the absorption of psoralen and isopsoralen, and increased the bioavailability of these compounds. Copyright