Inflammation-Induced Downregulation of Butyrate Uptake and Oxidation Is Not Caused by a Reduced Gene Expression

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• 作者：Leen Boesmans ; Meine Ramakers ; Ingrid Arijs ; Karen Windey ; Wiebe Vanhove ; Frans Schuit ; Paul Rutgeerts ; Kristin Verbeke and Vicky De Preter
• 刊名：Journal of Cellular Physiology
• 出版年：2015
• 出版时间：February 2015
• 年：2015
• 卷：230
• 期：2
• 页码：418-426
• 全文大小：1315K
• ISSN：1097-4652

文摘

In ulcerative colitis (UC) the butyrate metabolism is impaired, leading to energy-deficiency in the colonic cells. The effect of inflammation on the butyrate metabolism was investigated. HT-29 cells were incubated with pro-inflammatory cytokines (TNF- and/or IFN-) for 1 and 24 h. Cells were additionally stimulated with butyrate to investigate its anti-inflammatory potential. Butyrate uptake and oxidation were measured using ¹⁴C-labeled butyrate. Gene expression of the butyrate metabolism enzymes, interleukin 8 (IL-8; inflammatory marker) and villin-1 (VIL-1; epithelial cell damage marker) was measured via quantitative RT-PCR. Significantly increased IL-8 expression and decreased VIL-1 expression after 24 h incubation with TNF- and/or IFN- confirmed the presence of inflammation. These conditions induced a decrease of both butyrate uptake and oxidation, whereas the gene expression was not reduced. Simultaneous incubation with butyrate counteracted the reduced butyrate oxidation. In contrast, 1 h incubation with TNF- induced a significant increased IL-8 expression and decreased butyrate uptake. Incubation with TNF- and/or IFN- for 1 h did not induce cell damage nor influence butyrate oxidation. The inflammation-induced downregulation of the butyrate metabolism was not caused by a reduced gene expression, but appeared consequential to a decreased butyrate uptake. Increasing the luminal butyrate levels might have therapeutic potential in UC. J. Cell. Physiol. 230: 418–426, 2015.