Diversity-Oriented Synthesis of Natural-Product-like Libraries Containing a 3-Methylbenzofuran Moiety for the Discovery of New Chemical Elicitors
详细信息 查看全文
- 作者：Dr. Xingrui He ; Dr. Xia Chen ; Songbo Lin ; Xiaochang Mo ; Pengyong Zhou ; Zhihao Zhang ; Yaoyao Lu ; Yu Yang ; Haining Gu ; Prof. Zhicai Shang ; Prof. Yonggen Lou and Prof. Jun Wu
- 刊名：ChemistryOpen
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：6
- 期：1
- 页码：102-111
- 全文大小：2251K
- ISSN：2191-1363
文摘
Natural products are a major source of biological molecules. The 3-methylfuran scaffold is found in a variety of plant secondary metabolite chemical elicitors that confer host-plant resistance against insect pests. Herein, the diversity-oriented synthesis of a natural-product-like library is reported, in which the 3-methylfuran core is fused in an angular attachment to six common natural product scaffolds—coumarin, chalcone, flavone, flavonol, isoflavone and isoquinolinone. The structural diversity of this library is assessed computationally using cheminformatic analysis. Phenotypic high-throughput screening of β-glucuronidase activity uncovers several hits. Further in vivo screening confirms that these hits can induce resistance in rice to nymphs of the brown planthopper Nilaparvata lugens. This work validates the combination of diversity-oriented synthesis and high-throughput screening of β-glucuronidase activity as a strategy for discovering new chemical elicitors.