Profiling Functional and Biochemical Phenotypes of Circulating Tumor Cells Using a Two-Dimensional Sorting Device

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• 作者：Mahla Poudineh ; Dr. Mahmoud Labib ; Dr. Sharif Ahmed ; L. N. Matthew Nguyen ; Leyla Kermanshah ; Dr. Reza M. Mohamadi ; Prof. Edward H. Sargent and Prof. Shana O. Kelley
• 刊名：Angewandte Chemie International Edition
• 出版年：2017
• 出版时间：January 2, 2017
• 年：2017
• 卷：56
• 期：1
• 页码：163-168
• 全文大小：4007K
• ISSN：1521-3773

文摘

During cancer progression, tumors shed circulating tumor cells (CTCs) into the bloodstream. CTCs that originate from the same primary tumor can have heterogeneous phenotypes and, while some CTCs possess benign properties, others have high metastatic potential. Deconstructing the heterogeneity of CTCs is challenging and new methods are needed that can sort small numbers of cancer cells according to their phenotypic properties. Here we describe a new microfluidic approach that profiles, along two independent phenotypic axes, the behavior of heterogeneous cell subpopulations. Cancer cells are first profiled according to expression of a surface marker using a nanoparticle-enabled approach. Along the second dimension, these subsets are further separated into subpopulations corresponding to migration profiles generated in response to a chemotactic agent. We deploy this new technique and find a strong correlation between the surface expression and migration potential of CTCs present in blood from mice with xenografted tumors. This system provides an important new means to characterize functional diversity in circulating tumor cells.