Establishment of polyethylene-glycol-mediated protoplast transformation for Lecanicillium lecanii and development of virulence-enhanced strains against Aphis gossypii
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- 作者：Yan-Jun Zhang ; Ming Xie ; Xiao-Lin Zhang ; De-Liang Peng ; Wen-Bin Yu ; Qiang Li ; Qian Li ; Jin-Jin Zhao and Zhao-Rong Zhang
- 刊名：Pest Management Science
- 出版年：2016
- 出版时间：October 2016
- 年：2016
- 卷：72
- 期：10
- 页码：1951-1958
- 全文大小：422K
- ISSN：1526-4998
文摘
Lecanicillium lecanii has been developed as a biopesticide and used in biological control of several agricultural insects. To improve fungal virulence, an optimised polyethylene glycol (PEG)-mediated protoplast transformation system was established for L. lecanii. Pr1A-like cuticle-degrading protease gene (Cdep1) from Beauveria bassiana was transferred into L. lecanii, and its resulting activity against Aphis gossypii was assessed.