Overexpression of Jatropha curcas Defensin (JcDef) Enhances Sheath Blight Disease Resistance in Tobacco

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• 作者：Qingwei Wang ; Shuhua Zhu ; Yao Liu ; Rui Li ; Shancai Tan ; Shenghua Wang ; Lin Tang and Fang Chen
• 刊名：Journal of Phytopathology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：165
• 期：1
• 页码：15-21
• 全文大小：790K
• ISSN：1439-0434

文摘

Plant defensins are small, basic, cysteine-rich peptides, belonging to the antimicrobial peptide superfamily, commonly found in the plant kingdom. In this study, we cloned and characterized a plant defensin gene from Jatropha curcas (JcDef). JcDef carried conserved receptor binding sites and a cysteine motif, and it was phylogenetically grouped together with defensin Ec-AMP-D2-like in Elaeis guineensis. JcDef is localized to cytoplasm and highly expressed in young tissues with fast metabolism such as cotyledons and stem apexes. Transgenic expression of JcDef in tobacco showed enhanced resistance against sheath blight disease caused by R. solani, indicating the antibacterial function.