A structurally plastic ribonucleoprotein complex mediates post-transcriptional gene regulation in HIV-1

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• 作者：Jason D. Fernandes ; David S. Booth and Alan D. Frankel
• 刊名：Wiley Interdisciplinary Reviews: RNA
• 出版年：2016
• 出版时间：July/August 2016
• 年：2016
• 卷：7
• 期：4
• 页码：470-486
• 全文大小：1458K
• ISSN：1757-7012

文摘

HIV replication requires the nuclear export of essential, intron-containing viral RNAs. To facilitate export, HIV encodes the viral accessory protein Rev which binds unspliced and partially spliced viral RNAs and creates a ribonucleoprotein complex that recruits the cellular Chromosome maintenance factor 1 export machinery. Exporting RNAs in this manner bypasses the necessity for complete splicing as a prerequisite for mRNA export, and allows intron-containing RNAs to reach the cytoplasm intact for translation and virus packaging. Recent structural studies have revealed that this entire complex exhibits remarkable plasticity at many levels of organization, including RNA folding, protein–RNA recognition, multimer formation, and host factor recruitment. In this review, we explore each aspect of plasticity from structural, functional, and possible therapeutic viewpoints. WIREs RNA 2016, 7:470–486. doi: 10.1002/wrna.1342