Self-Assembled Dual Dye-Doped Nanosized Micelles for High-Contrast Up-Conversion Bioimaging

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• 作者：Sara Mattiello ; Angelo Monguzzi ; Jacopo Pedrini ; Mauro Sassi ; Chiara Villa ; Yvan Torrente ; Roberto Marotta ; Francesco Meinardi and Luca Beverina
• 刊名：Advanced Functional Materials
• 出版年：2016
• 出版时间：December 13, 2016
• 年：2016
• 卷：26
• 期：46
• 页码：8447-8454
• 全文大小：1429K
• ISSN：1616-3028

文摘

Sensitized triplet–triplet annihilation based photon up-conversion (TTA-UC) greatly improves the scope and applicability of fluorescence bioimaging by enabling anti-Stokes detection at low powers, thus eliminating the background autofluorescence and limiting the potential damage of the living tissues. Here the authors present a facile, one-step protocol to prepare dual dye-doped, TTA-UC active nanomicelles starting from the commercially available surfactant Kolliphor EL, a component of several FDA approved preparations. These nanosized micelles show an unprecedented up-conversion yield of 6.5% under 0.1 W cm⁻² excitation intensity in an aqueous, nondeaerated dispersion. The supramolecular architecture obtained preserves the embedded dyes from oxygen quenching, allowing satisfactory anti-Stokes fluorescence imaging of 3T3 cells. This is the first example of efficient multicomponent up-converters prepared using highly biocompatible materials approved by the international authority, paving the way for the development of new complex and multifunctional materials for advanced theranostics.