Gene expression profiles of human subcutaneous and visceral adipose-derived stem cells

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• 作者：Boyun Kim ; Boram Lee ; Mi-Kyung Kim ; Seung Pyo Gong ; Noh Hyun Park ; Hyun Hoon Chung ; Hee Seung Kim ; Jae Hong No ; Woong Yang Park ; Ae Kyung Park ; Jeong Mook Lim and Yong Sang Song
• 刊名：Cell Biochemistry and Function
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：34
• 期：8
• 页码：563-571
• 全文大小：605K
• ISSN：1099-0844

文摘

Subcutaneous and visceral adipose tissues show a different risk effect on metabolic disorders because they have distinct cellular properties. We isolated stem cells from the separate human adipose tissues to investigate that subcutaneous and visceral fat depots have metabolic differences. Adipose-derived stem cells (ASCs) were characterized by immunophenotype and differentiation potentials into adipogenic, osteogenic, and chondrogenic lineages. Although subcutaneous and visceral ASCs (S-ASC and V-ASC) express same surface markers (CD31⁻, CD34⁻, CD45⁻, CD73⁺, CD90⁺, and CD105⁺) and have differentiation potentials, S-ASCs had higher capacity to proliferate and to differentiate into adipogenic lineage than V-ASCs. Next, we identified that S-ASC and V-ASC were genetically distinct based on microarray analysis. Among a total of 810 genes detected in ASCs of both depots, the differentially expressed genes were involved in energy and lipid metabolism. These data show the existence of the intrinsic difference between S-ASC and V-ASC and suggest the differences of anatomically separated adipose tissue. On the basis of the differentially expressed gene profiles between S-ASC and V-ASC, we suggested significant evidence that adipose tissues originating from different anatomic regions are distinguished at the level of the undifferentiated stem cells such as mature adipocytes. V-ASCs had the upregulated clusters of genes related to lipid biosynthesis and metabolism. By contrast, S-ASCs highly expressed genes involved in DNA-dependent transcription, contributing to proliferation. We provide further insights for ASCs with the different origins to understand fat accumulation and distribution and a possibility of ASCs as a therapeutic target against metabolic disorders or cancer.