Characterization of MymA protein as a flavin-containing monooxygenase and as a target of isoniazid

详细信息    查看全文

• 作者：Iti Saraav ; Kirti Pandey ; Richa Misra ; Swati Singh ; Monika Sharma and Sadhna Sharma
• 刊名：Chemical Biology & Drug Design
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：89
• 期：1
• 页码：152-160
• 全文大小：987K
• ISSN：1747-0285

文摘

Tuberculosis is a global health problem especially with the emergence of drug-resistant Mycobacterium tuberculosis strains, creating an urgent need to identify new drug targets. The mycobacterial cell wall is an attractive target for chemotherapeutic agents. Gene products of mymA operon are known to be required for the maintenance of cell wall and play an important role in persistence, thus making them important drug targets. This study was undertaken to biochemically characterize the MymA as a flavin-containing monooxygenase (FMO). Our results established its enzymatic activity in vitro and found that the mycobacterial FMO requires NADPH and FAD as cofactors, similar to other characterized bacterial FMOs. The enzyme follows Michaelis–Menten kinetics to catalyze substrates such as trimethylamine and thiourea. We also propose that MymA could be one of the targets of the antituberculosis drug, isoniazid (INH), which is a cell wall inhibitor. Molecular docking studies revealed that INH targeted NADPH-binding site of the MymA. Further, experimental validation revealed that INH inhibits MymA with the ICb>50b> value of 4.9 μm. Thus, this study characterizes for the first time that MymA is a mycobacterial FMO, which may be a target of INH.