Histological and antioxidant responses in Rhamdia quelen sedated with propofol
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- 作者：Luciane Tourem Gressler ; Fernando Jonas Sutili ; Luiza Loebens ; Etiane Medianeira Hundertmark Saccol ; Tanise Silva Pê ; s ; Taylise Vey Parodi ; Sí ; lvio Teixeira da Costa ; Maria Amá ; lia Pavanato and Bernardo Baldisserotto
- 刊名：Aquaculture Research
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：47
- 期：7
- 页码：2297-2306
- 全文大小：275K
- ISSN：1365-2109
文摘
Morphometry of gills and antioxidant/oxidant status in gills, brain, liver and blood of Rhamdia quelen sedated with propofol were studied. The purpose was to investigate structural and functional responses upon administration of the drug in order to validate its use for the species. The fish were exposed to 0, 0.4 or 0.8 mg L−1 propofol for 1, 6 or 12 h, which are times normally used in live fish transport. Propofol induced an increase in chloride cell in the non-respiratory epithelium of the gill. Standard biochemical assays (thiobarbituric acid reactive substances and lipid hydroperoxides) indicated that the lowest concentration of propofol did not induce lipoperoxidation in gills, brain, liver or blood. The oxidative status of enzymatic (catalase, superoxide dismutase and glutathione-S-transferase) and non-enzymatic (non-protein thiols) antioxidants differed among the tested sites. Apart from the blood, superoxide dismutase was the enzyme to show the highest activity in the presence of propofol, which is known to possess antioxidant properties that resemble those of vitamin E. The absence of chloride cells in the respiratory epithelium (lamella) in association with the stability of the lamellar structure (i.e. lamellar area, total height of lamella and width of lamella) indicate that ion and oxygen uptake were preserved under propofol sedation. Thus, 0.4 mg L−1 propofol should be considered to sedate R. quelen during lengthy procedures, such as transport, for it was able to maintain ionic and respiratory homeostasis as well as prevent peroxidative damage in vital organs.