Cholesterol loading in vivo and in vitro alters extracellular matrix proteins production in smooth muscle cells

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• 作者：Rogelio Palomino-Morales ; Sonia Perales ; Carolina Torres ; Ana Linares and Maria Jose Alejandre
• 刊名：European Journal of Lipid Science and Technology
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：118
• 期：9
• 页码：1317-1325
• 全文大小：1447K
• ISSN：1438-9312

文摘

This study supports the SMC dedifferentiation in vitro model and increases our fundamental knowledge of plaque formation and remodeling. With this purpose, we isolated smooth muscle cells form aortic arch of chicks either fed under control or high cholesterol diets. From the former we obtained control SMCs (SMC−C) that were then loaded with cholesterol using cholesterol:methyl-β-cyclodextrin (CS) complexes, thus producing cholesterol loaded SMCs in vitro (SMC−C+CS).tained in vivo cholesterol loaded SMC cell cultures (SMC−Ch). We compared the effect of both cholesterol loading processes on extracellular matrix (ECM) production. The proline incorporation showed that collagen synthesis was higher in SMC−Ch and SMC−C+CS than in SMC−C cultures. Quantitative real-time PCR revealed that expression of extracellular matrix protein-related genes increased after in vitro and in vivo cholesterol loading with respect to unloaded cells, demonstrating a regulation at mRNA level. In addition, the expression of col3a1 and fibronectin genes was significantly higher in SMC−Ch than in SMC−Ch+CS cultures. We demonstrated that SMC can switch from contractile to synthetic state not only by cholesterol in vivo but also by cholesterol SMC loading in vitro.