Determination of benzo[a]pyrene in edible oils using phase-transfer-catalyst-assisted saponification and supramolecular solvent microextraction coupled to HPLC with fluorescence detection

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• 作者：Jin Wang ; Laping Liu ; Ludi Shi ; Tingquan Yi ; Yuxia Wen ; Juanli Wang and Shuhui Liu
• 刊名：Journal of Separation Science
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：40
• 期：2
• 页码：480-487
• 全文大小：357K
• ISSN：1615-9314

文摘

For the analysis of edible oils, saponification is well known as a useful method for eliminating oil matrices. The conventional approach is conducted with alcoholic alkali; it consumes a large volume of organic solvents and impedes the retrieval of analytes by microextraction. In this study, a low-organic-solvent-consuming method has been developed for the analysis of benzo[a]pyrene in edible oils by high-performance liquid chromatography with fluorescence detection. Sample treatment involves aqueous alkaline saponification, assisted by a phase-transfer catalyst, and selective in situ extraction of the analyte with a supramolecular solvent. Comparison of the chromatograms of the oil extracts obtained by different microextraction methods showed that the supramolecular solvent has a better clean-up effect for the unsaponifiable matter from oil matrices. The method offered excellent linearity over a range of 0.03– 5.0 ng mL⁻¹ (r > 0.999). Recovery rates varied from 94 to 102% (RSDs <5.0%). The detection limit and quantification limit were 0.06 and 0.19 μg kg⁻¹, respectively. The proposed method was applied for the analysis of 52 edible oils collected online in China; the analyte contents of 23 tested oil samples exceeded the maximum limit of 2 μg kg⁻¹ for benzo[a]pyrene set by the Commission Regulation of the European Union.