A Protein-Nanocellulose Paper for Sensing Copper Ions at the Nano- to Micromolar Level

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• 作者：Ramon Weishaupt ; Gilberto Siqueira ; Mark Schubert ; Michael M. Kä ; mpf ; Tanja Zimmermann ; Katharina Maniura-Weber and Greta Faccio
• 刊名：Advanced Functional Materials
• 出版年：2017
• 出版时间：January 26, 2017
• 年：2017
• 卷：27
• 期：4
• 全文大小：1809K
• ISSN：1616-3028

文摘

Tracing heavy metals is a crucial issue in both environmental and medical samples. In this work, a sensing biomolecule, the cyanobacterial C-phycocyanin (CPC), is integrated into a nanocellulose matrix, and with this, a biosensor for copper ions is developed. The assembly of CPC-functionalized nanocellulose into a red-fluorescent, copper-sensitive hybrid film “CySense”, enhances protein stability and facilitates the reuse and the regeneration of the sensor for several cycles over 7 days. CySense is suitable for the analysis of complex medical samples such as human serum filtrate. The reported biosensor reliably detects copper ion contents with a lower detection limit of 200 × 10⁻⁹m and an IC50 of 4.9 × 10⁻⁶m as changes in fluorescence emission intensity that can be measured with a fluorimeter or a microarray laser scanner.