Improved light microscopy counting method for accurately counting Plasmodium parasitemia and reticulocytemia
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- 作者：Caeul Lim ; Ligia Pereira ; Pritish Shardul ; Anjali Mascarenhas ; Jennifer Maki ; Jordan Rixon ; Kathryn Shaw-Saliba ; John White ; Maria Silveira ; Edwin Gomes ; Laura Chery ; Pradipsinh K. Rathod and Manoj T. Duraisingh
- 刊名：American Journal of Hematology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：91
- 期：8
- 页码：852-855
- 全文大小：700K
- ISSN：1096-8652
文摘
Even with the advances in molecular or automated methods for detection of red blood cells of interest (such as reticulocytes or parasitized cells), light microscopy continues to be the gold standard especially in laboratories with limited resources. The conventional method for determination of parasitemia and reticulocytemia uses a Miller reticle, a grid with squares of different sizes. However, this method is prone to errors if not used correctly and counts become inaccurate and highly time-consuming at low frequencies of target cells. In this report, we outline the correct guidelines to follow when using a reticle for counting, and present a new counting protocol that is a modified version of the conventional method for increased accuracy in the counting of low parasitemias and reticulocytemias. Am. J. Hematol. 91:852–855, 2016.