Quantitative Single-Cell mRNA Analysis in Hydrogel Beads

详细信息    查看全文

• 作者：Agata Rakszewska ; Rosa J. Stolper ; Anna B. Kolasa ; Dr. Aigars Piruska and Prof. Dr. Wilhelm T. S. Huck
• 刊名：Angewandte Chemie International Edition
• 出版年：2016
• 出版时间：June 1, 2016
• 年：2016
• 卷：55
• 期：23
• 页码：6698-6701
• 全文大小：2456K
• ISSN：1521-3773

文摘

In recent years, technologies capable of analyzing single cells have emerged that are transforming many fields of biological research. Herein we report how DNA-functionalized hydrogel beads can serve as a matrix to capture mRNA from lysed single cells. mRNA quantification free of pre-amplification bias is ensured by using padlock probes and rolling circle amplification followed by hybridization with fluorescent probes. The number of transcripts in individual cells is assessed by simply counting fluorescent dots inside gel beads. The method extends the potential of existing techniques and provides a general platform for capturing molecules of interest from single cells.