Immobilization of the Gas Signaling Molecule H2S by Radioisotopes: Detection, Quantification, and In Vivo Imaging

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• 作者：Dr. Swarbhanu Sarkar ; Yeong Su Ha ; Nisarg Soni ; Dr. Gwang Il An ; Woonghee Lee ; Min Hwan Kim ; Phuong Tu Huynh ; Heesu Ahn ; Dr. Nikunj Bhatt ; Dr. Yong Jin Lee ; Dr. Jung Young Kim ; Prof. Kwon Moo Park ; Prof. Isao Ishii ; Prof. Shin-Geol Kang and Prof. Jeongsoo Yoo
• 刊名：Angewandte Chemie
• 出版年：2016
• 出版时间：August 1, 2016
• 年：2016
• 卷：128
• 期：32
• 页码：9511-9516
• 全文大小：3930K
• ISSN：1521-3757

文摘

Hydrogen sulfide (H₂S) has multifunctional roles as a gas signaling molecule in living systems. However, the efficient detection and imaging of H₂S in live animals is very challenging. Herein, we report the first radioisotope-based immobilization technique for the detection, quantification, and in vivo imaging of endogenous H₂S. Macrocyclic ⁶⁴Cu complexes that instantly reacted with gaseous H₂S to form insoluble ⁶⁴CuS in a highly sensitive and selective manner were prepared. The H₂S concentration in biological samples was measured by a thin-layer radiochromatography method. When ⁶⁴Cu–cyclen was injected into mice, an elevated H₂S concentration in the inflamed paw was clearly visualized and quantified by Cerenkov luminescence and positron emission tomography (PET) imaging. PET imaging was also able to pinpoint increased H₂S levels in a millimeter-sized infarcted lesion of the rat heart.