Potentials of single-cell biology in identification and validation of disease biomarkers

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• 作者：Furong Niu ; Diane C. Wang ; Jiapei Lu ; Wei Wu and Xiangdong Wang
• 刊名：Journal of Cellular and Molecular Medicine
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：20
• 期：9
• 页码：1789-1795
• 全文大小：684K
• ISSN：1582-4934

文摘

Single-cell biology is considered a new approach to identify and validate disease-specific biomarkers. However, the concern raised by clinicians is how to apply single-cell measurements for clinical practice, translate the message of single-cell systems biology into clinical phenotype or explain alterations of single-cell gene sequencing and function in patient response to therapies. This study is to address the importance and necessity of single-cell gene sequencing in the identification and development of disease-specific biomarkers, the definition and significance of single-cell biology and single-cell systems biology in the understanding of single-cell full picture, the development and establishment of whole-cell models in the validation of targeted biological function and the figure and meaning of single-molecule imaging in single cell to trace intra-single-cell molecule expression, signal, interaction and location. We headline the important role of single-cell biology in the discovery and development of disease-specific biomarkers with a special emphasis on understanding single-cell biological functions, e.g. mechanical phenotypes, single-cell biology, heterogeneity and organization of genome function. We have reason to believe that such multi-dimensional, multi-layer, multi-crossing and stereoscopic single-cell biology definitely benefits the discovery and development of disease-specific biomarkers.