Multiresidue determination of 114 multiclass pesticides in flue-cured tobacco by solid-phase extraction coupled with gas chromatography and tandem mass spectrometry

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• 作者：Jianmin Cao ; Na Sun ; Weisong Yu ; Xueli Pang ; Yingnan Lin ; Fanyu Kong and Jun Qiu
• 刊名：Journal of Separation Science
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：39
• 期：23
• 页码：4629-4636
• 全文大小：590K
• ISSN：1615-9314

文摘

A sensitive and robust multiresidue method for the simultaneous analysis of 114 pesticides in tobacco was developed based on solid-phase extraction coupled with gas chromatography and tandem mass spectrometry. In this strategy, tobacco samples were extracted with acetonitrile and cleaned up with a multilayer solid-phase extraction cartridge Cleanert TPT using acetonitrile/toluene (3:1) as the elution solvent. Two internal standards of different polarity were used to meet simultaneous pesticides quantification demands in the tobacco matrix. Satisfactory linearity in the range of 10–500 ng/mL was obtained for all 114 pesticides with linear regression coefficients higher than 0.994. The limit of detection and limit of quantification values were 0.02–5.27 and 0.06–17.6 ng/g, respectively. For most of the pesticides, acceptable recoveries in the range of 70–120% and repeatabilities (relative standard deviation) of <11% were achieved at spiking levels of 20, 100, and 400 ng/g. Compared with the reported multiresidue analytical method, the proposed method provided a cleaner test solution with smaller amounts of pigments, fatty acids as well as other undesirable interferences. The development and validation of the high sensitivity, high selectivity, easy automation, and high-throughput analytical method meant that it could be successfully used for the determination of pesticides in tobacco samples.