Label-Free and Continuous-Flow Ferrohydrodynamic Separation of HeLa Cells and Blood Cells in Biocompatible Ferrofluids
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- 作者：Wujun Zhao ; Taotao Zhu ; Rui Cheng ; Yufei Liu ; Jian He ; Hong Qiu ; Lianchun Wang ; Tamas Nagy ; Troy D. Querec ; Elizabeth R. Unger and Leidong Mao
- 刊名：Advanced Functional Materials
- 出版年：2016
- 出版时间：June 14, 2016
- 年：2016
- 卷：26
- 期：22
- 页码：3990-3998
- 全文大小：2484K
- ISSN：1616-3028
文摘
In this study, a label-free, low-cost, and fast ferrohydrodynamic cell separation scheme is demonstrated using HeLa cells (an epithelial cell line) and red blood cells. The separation is based on cell size difference, and conducted in a custom-made biocompatible ferrofluid that retains the viability of cells during and after the assay for downstream analysis. The scheme offers moderate-throughput (≈106 cells h−1 for a single channel device) and extremely high recovery rate (>99%) without the use of any label. It is envisioned that this separation scheme will have clinical applications in settings where rapid cell enrichment and removal of contaminating blood will improve efficiency of screening and diagnosis such as cervical cancer screening based on mixed populations in exfoliated samples.