Improved ε-poly-l-lysine productivity partly resulting from rapid cell growth in cultures using a glucose-glycerol mixed carbon source
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- 作者：Xin Zeng ; Xu-Sheng Chen ; Xi-Dong Ren ; Liang Wang ; Yang Gao and Zhong-Gui Mao
- 刊名：Engineering in Life Sciences
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：16
- 期：5
- 页码：443-452
- 全文大小：518K
- ISSN：1618-2863
文摘
Productivity enhancements with mixed carbon sources are usually accompanied by simultaneous improvement of cell growth. However, whether the enhanced cell growth in mixed carbon sources influences the biochemical productivity of ε-poly-l-lysine (ε-PL) still remains unclear. In this study, we investigated the effect of growth rate on the ε-PL productivity in a glucose–glycerol mixed carbon source. Based on the typical ε-PL fed batch fermentation, chemostat culture and relevant physiological analyses were carried out. The ε-PL productivity was positively correlated to the growth rate ranging from 0.02 to 0.06 h−1. The primary metabolism activity was enhanced at higher growth rate, providing sufficient precursor l-lysine and energy for ε-PL production. Meanwhile, these two key elements were equally important for biomass production, which could be quickly produced when the cells were fast growing. In addition, rapid growth also strengthened the antioxidant capacity of cells to defend potential oxidative stress. The positive correlation between the growth rate and ε-PL productivity indicated that the improvement of ε-PL productivity in the mixed carbon source was partly attributed to the simultaneously enhanced cell growth. Information obtained may provide references for further studies on other secondary biochemicals’ production using mixed carbon sources.