β-Actin protein expression differs in the submandibular glands of male and female mice

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• 作者：Gang Chen ; Ye Zou ; Xuan Zhang ; Lingfei Xu ; Qiaoyun Hu ; Ting Li ; Chenjuan Yao ; Shali Yu ; Xiaoke Wang and Chun Wang
• 刊名：Cell Biology International
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：40
• 期：7
• 页码：779-786
• 全文大小：9359K
• ISSN：1095-8355

文摘

β-actin, a cytoskeletal protein, is the most widely used housekeeping gene. Although housekeeping genes are expressed in all tissues, the β-actin gene is expressed in certain cell types because of differential binding of transcriptional factors to the regulatory elements of the gene. The expression and localization of β-actin protein in the submandibular glands (SMG) of mice were investigated in this study, using Western blot analysis and immunohistochemistry. In ICR and C57BL/6J mice, the levels of β-actin protein in the SMG of females are significantly higher than those in the SMG of males. β-actin protein is majorly distributed in acinar cells of SMG. There is no significant difference in the expression level of β-actin protein between females and castrated males. After castrated male ICR mice are treated with 10 mg/kg/day testosterone propionate (TP) for 3 weeks, the levels of β-actin protein in SMG decrease. The numbers of duct per unit area increase, whereas the numbers of acinus per unit area decrease after TP administration. These data suggest that β-actin protein is mainly distributed in acinar cells of SMG and results in a marked sexual dimorphism in mice.