Fingerprinting of Peptides with a Large Channel of Bacteriophage Phi29 DNA Packaging Motor

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• 作者：Zhouxiang Ji ; Shaoying Wang ; Zhengyi Zhao ; Zhi Zhou ; Farzin Haque and Peixuan Guo
• 刊名：Small
• 出版年：2016
• 出版时间：September 7, 2016
• 年：2016
• 卷：12
• 期：33
• 页码：4572-4578
• 全文大小：886K
• ISSN：1613-6829

文摘

Nanopore technology has become a highly sensitive and powerful tool for single molecule sensing of chemicals and biopolymers. Protein pores have the advantages of size amenability, channel homogeneity, and fabrication reproducibility. But most well-studied protein pores for sensing are too small for passage of peptide analytes that are typically a few nanometers in dimension. The funnel-shaped channel of bacteriophage phi29 DNA packaging motor has previously been inserted into a lipid membrane to serve as a larger pore with a narrowest N-terminal constriction of 3.6 nm and a wider C-terminal end of 6 nm. Here, the utility of phi29 motor channel for fingerprinting of various peptides using single molecule electrophysiological assays is reported. The translocation of peptides is proved unequivocally by single molecule fluorescence imaging. Current blockage percentage and distinctive current signatures are used to distinguish peptides with high confidence. Each peptide generated one or two distinct current blockage peaks, serving as typical fingerprint for each peptide. The oligomeric states of peptides can also be studied in real time at single molecule level. The results demonstrate the potential for further development of phi29 motor channel for detection of disease-associated peptide biomarkers.