This study aims to simultaneously reduce biogas slurry nutrient and upgrade biogas via microalgal production. Marine microalgae were grown in a culture medium containing a mixture of seawater and biogas slurry with CO2 from synthetic biogas. The effects of different ratios of biogas slurry and various influent concentrations of CH4 in synthetic biogas on the growth of Nannochloropsis sp. were examined. Nutrient removal and biogas upgrading were also studied under different influent CH4 concentrations.