Surface Enhanced Raman Scattering and Gated Materials for Sensing Applications: The Ultrasensitive Detection of Mycoplasma and Cocaine
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- 作者：Mar Oroval ; Dr. Marc Coronado-Puchau ; Dr. Judith Langer ; Dr. Marta Norah Sanz-Ortiz ; Á ; ngela Ribes ; Dr. Elena Aznar ; Dr. Carmen Coll ; Dr. Marí ; a Dolores Marcos ; Dr. Fé ; lix Sancenó ; n ; Prof. Luis M. Liz-Marzá ; n and Prof. Ramó ; n Martí ; nez-Má ; ñ ; ez
- 刊名：Chemistry - A European Journal
- 出版年：2016
- 出版时间：September 12, 2016
- 年：2016
- 卷：22
- 期：38
- 页码：13488-13495
- 全文大小：1268K
- ISSN：1521-3765
文摘
We present herein a novel combination of gated mesoporous silica nanoparticles (MSNs) and surface-enhanced Raman scattering (SERS) for sensing applications. As a proof-of-concept, we show the design of a system comprising MSNs loaded with crystal violet (CV), a molecule with high Raman cross section acting as SERS reporter, and capped with either a suitable DNA sequence for the detection of Mycoplasma genomic DNA or with an aptamer that selectively coordinates cocaine. In both cases the presence of the corresponding target analyte in solution (i.e., genomic DNA or cocaine) resulted in the release of CV. CV delivery was detected by SERS upon adsorption on gold nanotriangles (AuNTs), which display an efficient electromagnetic field enhancement and a high colloidal stability. By using this novel procedure a limit of detection of at least 30 copies DNA per μL was determined for the detection of Mycoplasma genomic DNA, whereas cocaine was detected at concentrations as low as 10 nm.