Comparison of three whole genome amplification methods for detection of genomic aberrations in single cells

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• 作者：Elizabeth Normand ; Sadeem Qdaisat ; Weimin Bi ; Chad Shaw ; Ignatia Van den Veyver ; Arthur Beaudet ; Amy Breman
• 刊名：Prenatal Diagnosis
• 出版年：2016
• 出版时间：September 2016
• 年：2016
• 卷：36
• 期：9
• 页码：823-830
• 全文大小：476.2KB
• ISSN：1097-0223

文摘

Detection of genomic copy number abnormalities in a single cell using array comparative genomic hybridization (CGH) offers a promising non-invasive alternative for prenatal diagnosis. Our objective was to compare three commercially available whole-genome amplification (WGA) kits for their capacity to produce high quality DNA from single cells that is suitable for both molecular genotyping and array CGH.