Characterization of a rat orthotopic pancreatic head tumor model using three-dimensional and quantitative multi-parametric MRI

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• 作者：Ting Yin ; Ronald Peeters ; Yuanbo Feng ; Yewei Liu ; Jie Yu ; Steven Dymarkowski ; Uwe Himmelreich ; Raymond Oyen and Yicheng Ni
• 刊名：NMR in Biomedicine
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：30
• 期：2
• 全文大小：1242K
• ISSN：1099-1492

文摘

The purpose of this study was to investigate the reliability of 3D isotropic MRI and quantitative multi-parametric MRI characterization on an orthotopic pancreatic head tumor model in rats. 3D isotropic T₂-weighted MRI was performed as a routine for tumor longitudinal follow-up and volume estimation. Common bile duct diameter was measured from 3D multiplanar reconstruction. Quantitative multi-parametric measurements including pixel-wise T₂, T₁ relaxivity, apparent diffusion coefficient (ADC) and apparent diffusion kurtosis mapping were performed twice throughout tumor growth. Semi-quantitative and quantitative analyses based on an extended Tofts model were applied to region-of-interest-based dynamic contrast-enhanced imaging, followed by contrast ratio measurement on standard contrast-enhanced imaging. Moreover, low-level texture-based analysis was inspected for T₂, T₁, ADC and contrast ratio measurements. Results indicated that multi-parametric MRI showed good reproducibility for tumor characterization; the measurements were not affected by tumor growth. Tumor growth was further confirmed with histology examinations. To conclude, state-of-the-art clinical MRI techniques were translated to this preclinical tumor model with high reliability, and have paved the way for translational oncology studies on this tumor model.