Experimental-based material parameter identification of oocytes

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• 作者：Johannes Dittmann ; Dagmar Tö ; pfer and Markus Bö ; l
• 刊名：PAMM
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：16
• 期：1
• 页码：79-80
• 全文大小：88K
• ISSN：1617-7061

文摘

In comparison to other eukaryotic cells, mammalian oocytes are characterised by a relative high diameter allowing in turn a straightforward micromechanical testing to study their mechanical properties. The structure of mammalian oocytes is characterised by the so-called zona pellucida (ZP), a thick glycoprotein layer, surrounding the cells interior, the ooplasm. In contrast to other cells, where the load is mainly carried by inner cell structures, in case of oocytes a huge amount of external loads is carried by the ZP. Aim of this work is the determination of the mechanical properties of oocytes. Therefore, a micromechanical setup has been developed and installed on a microscope. Beside the determination of the force-strain relation during loading, the deformation of the oocytes has been recorded optically, too. Both, the force-strain curves and the optical recordings build the basis for a proper parameter identification technique based on the inverse finite element method. (