A Highly Photostable Hyperbranched Polyglycerol-Based NIR Fluorescence Nanoplatform for Mitochondria-Specific Cell Imaging
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- 作者：Chunhong Dong ; Zhongyun Liu ; Junqing Liu ; Changzhu Wu ; Falko Neumann ; Hanjie Wang ; Monika Schä ; fer-Korting ; Burkhard Kleuser ; Jin Chang ; Wenzhong Li ; Nan Ma and Rainer Haag
- 刊名：Advanced Healthcare Materials
- 出版年：2016
- 出版时间：September 7, 2016
- 年：2016
- 卷：5
- 期：17
- 页码：2214-2226
- 全文大小：4567K
- ISSN：2192-2659
文摘
Considering the critical role of mitochondria in the life and death of cells, non-invasive long-term tracking of mitochondria has attracted considerable interest. However, a high-performance mitochondria-specific labeling probe with high photostability is still lacking. Herein a highly photostable hyperbranched polyglycerol (hPG)-based near-infrared (NIR) quantum dots (QDs) nanoplatform is reported for mitochondria-specific cell imaging. Comprising NIR Zn-Cu-In-S/ZnS QDs as extremely photostable fluorescent labels and alkyl chain (C12)/triphenylphosphonium (TPP)-functionalized hPG derivatives as protective shell, the tailored QDs@hPG-C12/TPP nanoprobe with a hydrodynamic diameter of about 65 nm exhibits NIR fluorescence, excellent biocompatibility, good stability, and mitochondria-targeted ability. Cell uptake experiments demonstrate that QDs@hPG-C12/TPP displays a significantly enhanced uptake in HeLa cells compared to nontargeted QDs@hPG-C12. Further co-localization study indicates that the probe selectively targets mitochondria. Importantly, compared with commercial deep-red mitochondria dyes, QDs@hPG-C12/TPP possesses superior photostability under continuous laser irradiation, indicating great potential for long-term mitochondria labeling and tracking. Moreover, drug-loaded QDs@hPG-C12/TPP display an enhanced tumor cell killing efficacy compared to nontargeted drugs. This work could open the door to the construction of organelle-targeted multifunctional nanoplatforms for precise diagnosis and high-efficient tumor therapy.