Combining field-amplified sample stacking with moving reaction boundary electrophoresis on a paper chip for the preconcentration and separation of metal ions
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- 作者：Liangfei Ouyang ; Qian Liu and Heng Liang
- 刊名：Journal of Separation Science
- 出版年：2017
- 出版时间：February 2017
- 年：2017
- 卷：40
- 期：3
- 页码：789-797
- 全文大小：938K
- ISSN：1615-9314
文摘
A common drawback of paper-based separation devices is their poor detection limit. In this study, we combined field-amplified sample stacking with moving reaction boundary electrophoresis on a paper chip with six array channels for the parallel separation and concentration of multiple samples. With a new hyphenated technique, the brown I2 from the Fe3+/I− oxidation–reduction reaction emerged near the boundary between the dilute ethylene diamine tetraacetic acid and potassium iodide and highly concentrated KCl solutions. For the separation and concentration of three components, Cr3+, Cu2+, and Fe3+, the Fe3+ detection limit was improved at least 266-fold by comparing the hyphenated technique with moving reaction boundary electrophoresis. The detection limit of Fe3+ was found to be as low as 0.34 ng (20 μM) on the paper chip. We also demonstrated the analysis of a real sample of four metal ions, with detection limits as follows: 0.16 μg Cr3+, 1.5 μg Ni2+, 0.64 μg Cu2+, and 1.5 μg Co2+. The synergy of field-amplified sample stacking and moving reaction boundary electrophoresis in the micron paper-based array channels dramatically improved the detection limit and throughput of paper-based electrophoresis.