Effect of feeder free poly(lactide-co-glycolide) scaffolds on morphology, proliferation, and pluripotency of mouse embryonic stem cells

详细信息    查看全文

• 作者：Andrea G. Galuppo ; Pedro C. Chagastelles ; Douglas Gamba ; Daikelly B. Iglesias ; Laura E. Sperling ; Janine Machado ; J&eacute ; ssica F. T. C. Petry ; Joachim Wendorff ; Cesar L. Petzhold and Patricia Pranke
• 刊名：Journal of Biomedical Materials Research Part A
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：105
• 期：2
• 页码：424-432
• 全文大小：598K
• ISSN：1552-4965

文摘

The aim of the study has been to evaluate the morphology, proliferation, and pluripotency maintenance of mouse embryonic stem cells (mESCs) cultivated on poly(lactic-co-glycolic acid) scaffolds. The scaffolds were hydrolyzed with NaOH (treated) and nonhydrolyzed (untreated). Morphological and mechanical characterization of the scaffolds was performed. mESC were evaluated for cell viability, cytotoxicity, expression of pluripotency markers, colony morphology, and overall distribution. The treatment generated a reduction in the hydrophobic characteristics of the scaffolds, leading to a higher wettability compared to the untreated group. The viability, cytotoxicity, number of colonies, and the thickness of the cell layer presented similar results between the scaffold groups. The viability test showed that it was possible to cultivate the mESCs on the scaffolds. The cytotoxicity analysis showed that the PLGA scaffolds were not harmful for the cells. The cells maintained the expression of the pluripotency markers Oct4 and Sox2. The number of colonies and the thickness of the cell layer on the scaffold showed that they were not able to colonize the entire volume of the scaffolds. The area occupied by the mESCs was the same between the treated and untreated groups after 14 days in culture. It is possible to conclude that both conditions are equally suitable for maintaining mESC culture.