Human mesenchymal stromal cells response to biomimetic octacalcium phosphate containing strontium

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• 作者：Zeinab Tahmasebi Birgani ; Angad Malhotra ; Clemens A. van Blitterswijk and Pamela Habibovic
• 刊名：Journal of Biomedical Materials Research Part A
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：104
• 期：8
• 页码：1946-1960
• 全文大小：928K
• ISSN：1552-4965

文摘

The incorporation of bioinorganics into synthetic biomaterials is a promising approach to improve the biological performance of bone graft substitutes, while still retaining their synthetic nature. Among these bioinorganics, strontium ions (Sr²⁺) have reported enhanced bone formation, and a reduced risk of bone fractures. While previous results have been encouraging, more detailed studies are needed to further develop specific applications. This study demonstrates the effects of Sr²⁺ on the osteogenic differentiation of human mesenchymal stromal cells (hMSCs) when introduced as either a dissolved salt, or incorporated into biomimetic calcium phosphate (CaP) coatings. Upon attachment, hMSCs seeded in the presence of higher Sr²⁺ concentrations presented with a more elongated shape as compared to the controls without Sr²⁺. Both Sr²⁺ as a dissolved salt in the medium, or incorporated into CaP coatings, positively influenced hMSC alkaline phosphatase (ALP) activity in a dose-dependent manner. At the mRNA level, the expression of osteogenic markers ALP, bone sialoprotein, bone morphogenetic protein 2, osteopontin, and osteoclacin were increased in the presence of Sr²⁺, independent of the delivery method. Overall, this study demonstrates the positive effects of strontium on the osteogenic differentiation of human MSCs, and supports the use of strontium-incorporated CaPs for bone regeneration applications.