Measurement of protein size in concentrated solutions by small angle X-ray scattering

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• 作者：Jun Liu ; Zhihong Li ; Yanru Wei ; Wenjia Wang ; Bing Wang ; Hongli Liang and Yuxi Gao
• 刊名：Protein Science
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：25
• 期：8
• 页码：1385-1389
• 全文大小：266K
• ISSN：1469-896X

文摘

By simulations on the distance distribution function (DDF) derived from small angle X-ray scattering (SAXS) theoretical data of a dense monodisperse system, we found a quantitative mathematical correlation between the apparent size of a spherically symmetric (or nearly spherically symmetric) homogenous particle and the concentration of the solution. SAXS experiments on protein solutions of human hemoglobin and horse myoglobin validated the correlation. This gives a new method to determine, from the SAXS DDF, the size of spherically symmetric (or nearly spherically symmetric) particles of a dense monodisperse system, specifically for protein solutions with interference effects.