Expression profiles of two thaumatin-like protein (TmTLP) genes in responses to various micro-organisms from Tenebrio molitor

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• 作者：Dong Hyun Kim ; Mi Young Noh ; Ki Beom Park and Yong Hun Jo
• 刊名：Entomological Research
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：47
• 期：1
• 页码：35-40
• 全文大小：349K
• ISSN：1748-5967

文摘

It is well known that anti-fungal thaumatin-like proteins (TLPs) play important roles in plants. Here, we investigated the expression analysis of thaumatin-like protein genes TmTLPs in response to various pathogens in Tenebrio molitor. Developmental expression patterns of TmTLPs show that TmTLPs are highly expressed in the early pupal and adult stages. Furthermore, tissue-specific expression patterns of TmTLPs indicate that TmTLP1 is highly expressed in the integument and gut of last instar larvae and the integument and Malpighian tubules of 5-day old adults. In contrast, TmTLP2 is highly expressed in the gut of both last instar larvae and 5-day old adults. We hypothesize that the expression of TmTLP genes in developmental stages may be related to molting and body remodeling stresses. In addition, the induction patterns of TmTLP genes indicate that TmTLPs were slightly induced by Escherichia coli, Staphylococcus aureus, Candida albicans and Listeria monocytogenes. Furthermore, TmTLP1 and TmTLP2 were strongly induced in response to E. coli at 9 h post-injection and L. monocytogenes at 3 h post-injection. Our results suggest that TmTLPs may possess antimicrobial functions in T. molitor.