Effect of Siam weed extract and its bioactive component scutellarein tetramethyl ether on anti-inflammatory activity through NF-¦ÊB pathway

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• 作者：Hataichanok Pandith^a ; ^b ; Xiaobo Zhang^a ; Suchitra Thongpraditchote^c ; Yuvadee Wongkrajang^c ; Wandee Gritsanapan^b ; ^{wandee.gri@mahidol.ac.th} ; Seung Joon Baek^a ; ^{sbaek2@utk.edu}
• 关键词：COX-2 ; Cyclooxygenase-2 ; I¦ÊB¦Á ; Inhibitory-kappa-B-alpha ; IKK¦Á/¦Â ; I¦ÊB kinase complex alpha/beta ; iNOS ; Inducible nitro oxide synthase ; LPS ; Lipopolysaccharide ; NF-¦ÊB ; Nuclear factor-kappaB ; NO ; Nitric-oxide ; PGE2 ; Prostaglandin E2 ; RT-PCR ; Reverse tr
• 刊名：Journal of Ethnopharmacology
• 出版年：2013
• 出版时间：20 May, 2013
• 年：2013
• 卷：147
• 期：2
• 页码：434-441
• 全文大小：1078 K

文摘

Ethnopharmacological relevance

Siam weed (Chromolaena odorata (L.) King and Robinson) is a medicinal herb used for wound healing and inflammation-related diseases.

Aim of the study

In this study, we evaluated the molecular mechanism by which Siam weed extract (SWE) and its bioactive components, scutellarein tetramethyl ether (scu), stigmasterol, and isosakuranetin affect anti-inflammatory activity.

Materials and methods

The expression of several inflammatory proteins in RAW 264.7 (murine) macrophages was assessed by Western blot and reverse transcription-polymerase chain reaction (RT-PCR). Biochemical assays including prostaglandin E₂ (PGE₂) and nitric-oxide (NO) quantification were performed. Luciferase promoter activity and immunocytochemistry of Nuclear factor-¦ÊB (NF-¦ÊB) were investigated.

Results

Cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) are critical pro-inflammatory proteins. The level of protein and mRNA expression of these enzymes induced by lipopolysaccharide (LPS) was dramatically suppressed by treatment with SWE, scu, or stigmasterol compounds in a dose-dependent manner. They also reduced PGE₂ and NO release. We further analyzed the NF-¦ÊB pathway and found that the scu compound suppressed I¦ÊB kinase complex alpha/beta (IKK¦Á/¦Â) and Inhibitory-kappa-B-alpha (I¦ÊB¦Á), thereby suppressing COX-2 and iNOS expression.

Conclusion

This is the first report of the anti-inflammatory molecular mechanism in SWE and/or its bioactive component scu, indicating alteration NF-¦ÊB pathway and further providing potential uses in the treatment of inflammatory-related diseases.