Poly-3-amino-1,2,4-triazole-5-thiol (P(AT)) was coated on a glassy carbon electrode.
Electrochemical DNA biosensor was prepared by adsorption of dsDNA onto P(AT) modified electrode.
Guanine oxidation signals were linearly proportional to the concentration of BNN-17 in the range of 0.213–32.03 μmol L− 1.
BNN-17 and dsDNA interaction was investigated using DPV, UV–vis, spectroscopy, and molecular docking methods.