Let G be a connected graph with order n and 5c578785128" title="Click to view the MathML source">D(G) be the distance matrix of G . Suppose that λ1(D(G))≥⋯≥λn(D(G)) are the distance spectra of G . In this paper, we characterize the graphs with , where α is the smallest root of x3−x2−3x+1=0, and . Furthermore, we show that the graphs with are determined by their D-spectrum.