Because of its potential retro-conversion to androgenic steroids, 3尾-Adiol cannot be used 鈥渋n vivo鈥? thus, the aims of this study were to investigate the capability of four ligands of ER尾 (raloxifen, tamoxifen, genistein and curcumin) to counteract PC progression by mimicking the 3尾-Adiol activity.
Our results demonstrated that raloxifen, tamoxifen, genistein and curcumin decreased DU145 and PC3 cell proliferation in a dose-dependent manner; in addition, all four compounds significantly decreased the detachment of cells seeded on laminin or fibronectin. Moreover, raloxifen, tamoxifen, genistein and curcumin-treated DU145 and PC3 cells showed a significant decrease in cell migration. Notably, all these effects were reversed by the anti-estrogen, ICI 182,780, suggesting that their actions are mediated by the estrogenic pathway, via the ER尾, the only isoform present in these PCs.
In conclusion, these data demonstrate that by selectively activating the ER尾, raloxifen, tamoxifen, genistein and curcumin inhibit human PC cells proliferation and migration favoring cell adesion. These synthetic and natural modulators of ER action may exert a potent protective activity against the progression of PC even in its androgen-independent status.